Enzymes Lab

Lab: Enzymes Experiment 1: Effect of pH on Enzyme military action Date Objective: To study the matter of pH on enzymatic operation. Material Required: 1. Catalase enzyme 2. water system system2 3. Scaled Beaker 4. Dropper 5. Acidic stem 6. Basic solution theory Does change in pH affects an enzymatic operation? scrutiny of Hypothesis Enzyme may stick come forward unnatural by various factors. pH is also one out of these? It could be analyzed via introduction of change in pH specialize via addition of contrary dot and base in reaction mixture. For instance, in the reaction mixture of catalase with its substrate such as; H2O2. The reaction of Catalase enzyme on its substrate might be represented as follows: H2O2=H2O+1/2O2 If pH exit affect its activity thusly due to change in O2 production the direct of spume would also change. information entreaty Data might be collected via measurement of the take aim of foam in the scaled beaker. turn off 1: Effect of pH on Enzyme activity Tube| pH| Level of foam (in mm.)| | | 1| 2| 4| | | 2| 14| 1| | | 3| 8| 102| | | Data epitome Data analysis may be done via conclusion a relation between changed pH restrict and level of O2 produced in this way. Result According to the data, there is reduction in activity of enzyme in acidic (pH = 2) and highly basic condition (pH = 14) due to slight foam (means less O2) production.

But, at roughly basic condition (pH=8) the activity of enzyme is highest. Graphical Representation Enzyme activity (y axis) depends on judge of p H (x-axis) hence, there might be observed a! flower in graph where the value of enzymatic activity is maximum. O 8 Discussion The have-to doe with of pH on enzymatic activity might be due to change in the ionisation of amino acid residues and disruption in the non-covalent interactions. Conclusion Hence; it could be observed that our hypothesis that pH might also...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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